Cells, Free Full-Text

Olive flounder (Paralichthys olivaceus) muscle satellite cells (OFMCs) were obtained by enzymatic primary cell isolation and the explant method. Enzymatic isolation yielded cells that reached 80% confluence within 8 days, compared to 15 days for the explant method. Optimal OFMC growth was observed in 20% fetal bovine serum at 28 °C with 0.8 mM CaCl2 and the basic fibroblast growth factor (BFGF) to enhance cell growth. OFMCs have become permanent cell lines through the spontaneous immortalization crisis at the 20th passage. Olive flounder skeletal muscle myoblasts were induced into a mitogen-poor medium containing 2% horse serum for differentiation; they fused to form multinucleate myotubes. The results indicated complete differentiation of myoblasts into myotubes; we also detected the expression of the myogenic regulatory factors myoD, myogenin, and desmin. Upregulation (Myogenin, desmin) and downregulation (MyoD) of muscle regulation factors confirmed the differentiation in OFMCs.

An illustration of the full-duplex cell-free massive MIMO system.

Scheme of cell free cloning using DMF. ( A ) The full length construct

Postbiotics-parabiotics: the new horizons in microbial biotherapy and functional foods, Microbial Cell Factories

IJMS, Free Full-Text

Viruses, Free Full-Text

Towards reproducible cell-free systems

Iec/Ts 62886 Get File - Colaboratory

Mol Imaging Radionucl Ther on X: The Role of a Bone SPECT/CT Scan in the Follow-up of a Solitary Bone Lesion in a Patient with Langerhans' Cell Histiocytosis You can see the

Streptomyces cell-free systems for natural product discovery and engineering - ScienceDirect

JCM, Free Full-Text

Cells, Free Full-Text

Cell-free DNA tissues of origin by methylation profiling reveals significant cell, tissue, and organ-specific injury related to COVID-19 severity - ScienceDirect

Nature Reviews Molecular Cell Biology on X: From May cover Challenges and directions in studying cell–cell communication by #ExtracellularVesicles @DaveCarter1234 @guillaume_niel @VaderPieter Clayton Lambert & Raposo #EVsAreCool FREE pdf: https

Cell Circuits and Complex Tissues